Improved inverse PCR scheme for metagenome walking
نویسندگان
چکیده
منابع مشابه
Improved inverse PCR scheme for metagenome walking.
Inverse PCR has been used for the recovery of genome regions flanking a known sequence, although its application to metagenome walking is limited due to inefficient amplification from low copy number fragments. Here we present an improved inverse PCR scheme that enables walking of rare fragments in environmental metagenomes. Our scheme includes the following steps: (i) inverse PCR in which one ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2006
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112210