Improved inverse PCR scheme for metagenome walking

Improved inverse PCR scheme for metagenome walking.

Inverse PCR has been used for the recovery of genome regions flanking a known sequence, although its application to metagenome walking is limited due to inefficient amplification from low copy number fragments. Here we present an improved inverse PCR scheme that enables walking of rare fragments in environmental metagenomes. Our scheme includes the following steps: (i) inverse PCR in which one ...

An improved PCR method for walking in uncloned genomic DNA.

Several PCR-based methods are available for walking from a known region to an unknown region in cloned or uncloned genomic DNA. The methods are of three types: inverse PCR (1), randomly primed PCR (2) and adaptor ligation PCR (3-6). However, these methods have not been generally applied to walking in uncloned genomic DNA because they are either complicated or inefficient. Recent improvements to...

Splinkerettes--improved vectorettes for greater efficiency in PCR walking.

Efficient Codes for Inverse Dynamics During Walking

Efficient codes have been used effectively in both computer science and neuroscience to better understand the information processing in visual and auditory encoding and discrimination tasks. In this paper, we explore the use of efficient codes for representing information relevant to human movements during locomotion. Specifically, we apply motion capture data to a physical model of the human s...

Improved PCR-walking for large-scale isolation of plant T-DNA borders.

only one sequencing reaction. Instead of using ethanol precipitation after the first PCR, one can use filtration methods in a 96-well format with QIAquick 96 (Qiagen GmbH, Hilden, Germany) or NucleoSpin Multi-96 (Macherey-Nagel GmbH, Düren, Germany). These methods remove the PCR primers more efficiently and resulted in a remarkable reduction of amplification peaks in the intron sequences. How...